Longevindex

Protocols

Biohacking Protocols

Quick-reference protocols with links to full guides. Learn the science first, then follow the protocol.

Infrared Sauna Protocol

Strong Evidence

Huberman and Rhonda Patrick's most-cited longevity intervention. 140°F+ for 20+ minutes.

Frequency: 4–7× per weekDuration: 20–30 minutes
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Cold Exposure Protocol

Moderate Evidence

Huberman's minimum effective dose in uncomfortably cold water.

Frequency: 3–5× per weekDuration: 11 min total/week
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Sleep Optimization Protocol

Strong Evidence

Temperature, light, and tracking. The foundation of every biohack.

Frequency: NightlyDuration: 7–9 hours
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Red Light Therapy Protocol

Moderate Evidence

Morning photobiomodulation at 660nm + 850nm.

Frequency: DailyDuration: 10–20 minutes
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HRV Recovery Protocol

Moderate Evidence

Train based on recovery, not motivation.

Frequency: Daily monitoringDuration: Ongoing
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CGM Metabolic Protocol

Strong Evidence

Personalized nutrition through real-time glucose data.

Frequency: 14-day cyclesDuration: 1–3 months learning
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NAD+ Longevity Protocol

Emerging Evidence

NR or NMN with TMG methyl donor support.

Frequency: DailyDuration: Ongoing
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Creatine Protocol

Strong Evidence

5g monohydrate for muscle, brain, and healthy aging.

Frequency: DailyDuration: Ongoing
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Magnesium Sleep Protocol

Moderate Evidence

200–400mg glycinate 30–60 min before bed.

Frequency: DailyDuration: Ongoing
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Blueprint Core Stack

Moderate Evidence

Bryan Johnson's evidence-based essentials, not the full 100-pill stack.

Frequency: DailyDuration: Lifestyle
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Zone 2 Cardio Protocol

Strong Evidence

Peter Attia's mitochondrial foundation: conversational pace cardio.

Frequency: 4–5× per weekDuration: 45–60 min
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Breathwork Protocol

Moderate Evidence

Box breathing, Wim Hof, and physiological sigh for nervous system control.

Frequency: DailyDuration: 5–15 minutes
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Foundation Supplement Stack

Strong Evidence

Creatine, magnesium, vitamin D+K2, omega-3. The tier-1 stack.

Frequency: DailyDuration: Ongoing
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Contrast Therapy Protocol

Moderate Evidence

Frequency: 2–4× per weekDuration: Sauna 20 min, cold 3 min × 3
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Intermittent Fasting Protocol

Moderate Evidence

Dave Asprey's foundational metabolic biohack: 16:8 with fat-fueled mornings.

Frequency: DailyDuration: 16-hour fast
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Bulletproof Coffee Protocol

Moderate Evidence

Coffee + C8 MCT + grass-fed butter for ketone-fueled focus.

Frequency: Daily (morning)Duration: Replaces breakfast
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Vagus Nerve Toning Protocol

Moderate Evidence

Cold face immersion, slow breathing, and humming for nervous system resilience.

Frequency: DailyDuration: 5–10 minutes
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Neurofeedback Protocol

Moderate Evidence

EEG brain training for focus, calm, and meditation depth.

Frequency: 2–3× per weekDuration: 30–60 min sessions
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Huberman Sleep Stack

Emerging Evidence

50mg apigenin + magnesium + theanine for sleep onset support.

Frequency: NightlyDuration: 30–60 min before bed
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Lion's Mane Nootropic Protocol

Moderate Evidence

500–1000mg dual-extract lion's mane for cognitive support.

Frequency: DailyDuration: 4–8 weeks minimum
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Ashwagandha Stress Protocol

Moderate Evidence

600mg KSM-66 for cortisol reduction and stress resilience.

Frequency: DailyDuration: 8–12 weeks
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Blood Panel Baseline Protocol

Strong Evidence

Comprehensive biomarker testing with ApoB, insulin, and thyroid.

Frequency: 2× per yearDuration: Lifelong
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BPC-157 Recovery Protocol

Emerging Evidence

250–500mcg subcutaneous for tissue and gut healing.

Frequency: DailyDuration: 4–8 week cycles
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TB-500 Recovery Protocol

Emerging Evidence

2–2.5mg subcutaneous for tissue migration and joint recovery.

Frequency: 2× per weekDuration: 4–6 week loading
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Glycine Sleep Protocol

Moderate Evidence

3g glycine powder 30–60 min before bed for core temperature drop.

Frequency: NightlyDuration: Ongoing
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Circadian Rhythm Protocol

Strong Evidence

Morning sunlight within 60 min of waking, dim lights after sunset.

Frequency: DailyDuration: Lifestyle
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Grounding Protocol

Emerging Evidence

Barefoot outdoor contact or indoor grounding mat during sleep/work.

Frequency: DailyDuration: 20–60 minutes
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Urolithin A Longevity Protocol

Moderate Evidence

500mg urolithin A for mitophagy and mitochondrial health.

Frequency: DailyDuration: 4+ months
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Collagen Peptides Protocol

Moderate Evidence

10–15g hydrolyzed collagen in morning coffee or smoothie.

Frequency: DailyDuration: 8–12 weeks
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Blue Light Blocking Protocol

Moderate Evidence

Amber lenses plus dimmed overhead lights for melatonin protection.

Frequency: NightlyDuration: 1–2 hours before bed
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Epitalon Longevity Cycle

Emerging Evidence

5–10mg subcutaneous for pineal and telomerase support.

Frequency: Daily during cycleDuration: 10–20 days, 2× per year
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Huberman Sleep Stack (Full)

Emerging Evidence

Apigenin 50mg + magnesium 200–400mg + theanine 100–200mg.

Frequency: NightlyDuration: 30–60 min before bed
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D3 + K2 Protocol

Moderate Evidence

2000–5000 IU D3 + 100–200mcg K2 with fat-containing meal.

Frequency: DailyDuration: Ongoing
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Mouth Tape Nasal Breathing

Emerging Evidence

Porous vertical tape strip for nasal breathing and snoring reduction.

Frequency: NightlyDuration: Ongoing
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Berberine Metabolic Protocol

Moderate Evidence

500mg berberine per meal for glucose control and AMPK activation.

Frequency: 3× daily with mealsDuration: 8–12 week cycles
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Rhodiola Morning Protocol

Moderate Evidence

200–400mg standardized extract before breakfast for calm energy.

Frequency: Daily (AM only)Duration: 4–8 weeks
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NAC Glutathione Protocol

Moderate Evidence

600–1200mg NAC on empty stomach for antioxidant and liver support.

Frequency: DailyDuration: Ongoing
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CoQ10 Mitochondrial Protocol

Moderate Evidence

100–400mg ubiquinol with fatty meal, especially if on statins.

Frequency: DailyDuration: Ongoing
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Tongkat Ali Testosterone Protocol

Moderate Evidence

200–400mg standardized extract with baseline and follow-up bloodwork.

Frequency: DailyDuration: 8–12 weeks
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LMNT Electrolyte Protocol

Strong Evidence

1000mg sodium packet during fasting, sauna, or heavy training.

Frequency: As neededDuration: Ongoing
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Oura vs WHOOP Decision Guide

Strong Evidence

Compare sleep tracking, strain coaching, and subscription costs.

Frequency: One-time researchDuration: Before purchase
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Zinc Immune Protocol

Moderate Evidence

15–30mg zinc picolinate with food for immune and hormonal support.

Frequency: DailyDuration: Ongoing or seasonal
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Probiotic Gut Protocol

Moderate Evidence

10–50B CFU of clinically studied strains with prebiotic fiber.

Frequency: DailyDuration: 4–12 weeks
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Cryotherapy Protocol

Moderate Evidence

Whole-body cryo or ice bath for recovery and norepinephrine boost.

Frequency: 2–4× per weekDuration: 2–5 min per session
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Spermidine Autophagy Protocol

Emerging Evidence

1–6mg spermidine from wheat germ extract for cellular cleanup.

Frequency: DailyDuration: Ongoing
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Shilajit Vitality Protocol

Emerging Evidence

250–500mg purified shilajit resin for energy and testosterone support.

Frequency: DailyDuration: 8–12 weeks
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Peptides Introduction Protocol

Emerging Evidence

Understand peptide classes, sourcing, and safety before starting.

Frequency: Varies by peptideDuration: Education first
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HBOT Recovery Protocol

Moderate Evidence

Hyperbaric oxygen at 1.5–2.0 ATA for tissue repair and recovery.

Frequency: 1–3× per weekDuration: 60–90 min sessions
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Omega-3 Protocol

Strong Evidence

2–3g combined EPA+DHA for cardiovascular and brain health.

Frequency: DailyDuration: Ongoing
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Vitamin D Protocol

Strong Evidence

2000–5000 IU D3 with K2, test blood levels twice yearly.

Frequency: DailyDuration: Ongoing
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Gut Microbiome Protocol

Moderate Evidence

Diverse fiber, fermented foods, and targeted testing.

Frequency: DailyDuration: Lifestyle
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Testosterone Optimization Protocol

Moderate Evidence

Sleep, lifting, zinc, tongkat ali, and baseline blood panels.

Frequency: Daily + bloodworkDuration: 8–12 weeks
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Glutathione Support Protocol

Moderate Evidence

NAC, glycine, and sulfur-rich foods for master antioxidant production.

Frequency: DailyDuration: Ongoing
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Environmental Health Protocol

Moderate Evidence

Air purification, water filtration, and toxin reduction at home.

Frequency: DailyDuration: Lifestyle
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Nootropics Starter Protocol

Emerging Evidence

Begin with lion's mane, L-theanine, and caffeine before advanced stacks.

Frequency: DailyDuration: 4–8 weeks
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AG1 Daily Greens Protocol

Moderate Evidence

One scoop greens powder in water each morning for micronutrient coverage.

Frequency: DailyDuration: Ongoing
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Sleep Cooling Protocol

Strong Evidence

65–68°F bedroom, warm bath 90 min pre-bed, active cooling if needed.

Frequency: NightlyDuration: Ongoing
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Fisetin Senolytic Pulse

Emerging Evidence

1000mg fisetin/day for 2 days to clear senescent cells.

Frequency: 2–3 day pulsesDuration: Monthly or quarterly
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Methylene Blue Micro-Dose

Emerging Evidence

0.5–4mg pharma-grade methylene blue — never with SSRIs.

Frequency: DailyDuration: 4–8 week cycles
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Home Water Filtration

Strong Evidence

Test tap water, install RO or gravity filter for PFAS and lead removal.

Frequency: DailyDuration: Ongoing
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GHK-Cu Peptide Protocol

Moderate Evidence

1–2mg subQ or topical 1–2% serum for skin and tissue repair.

Frequency: Daily or EODDuration: 4–8 weeks
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Stem Cell Therapy Overview

Emerging Evidence

Understand stem cell types, clinics, and realistic outcome expectations.

Frequency: VariesDuration: Clinical consultation
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